منابع مشابه
Solubilization and purification of recombinant modified C-reactive protein from inclusion bodies using reversible anhydride modification.
The precise function of C-reactive protein (CRP) as a regulator of inflammation in health and disease continues to evolve. The true understanding of its role in host defense responses has been hampered by numerous reports of comparable systems with contradictory interpretations of CRP as a stimulator, suppressor, or benign contributor to such processes. These discrepancies may be explained in p...
متن کاملInclusion bodies in trachoma.
THE epithelial-cell inclusion, which is characteristically found in the early stages of trachoma was first described by Halberstaedter and Prowazek (1907). Since that time, a considerable literature concerning the nature of these bodies has accumulated. It is generally conceded that they consist of virus particles together with material elaborated by the virus and/or produced by the parasitized...
متن کاملRecovery of soluble, active recombinant protein from inclusion bodies.
In summary, three conditions need to be fulfilled to successfully sequence directly on large DNA templates. First, the amount of DNA in the sequencing reaction must be in the range of 3–4 μg. In this way, more DNA molecules will be available for priming during the first cycles of the PCR. Second, the DNA must be highly pure because relatively large amounts of DNA must be added in the sequencing...
متن کاملPackaging protein drugs as bacterial inclusion bodies for therapeutic applications
A growing number of insights on the biology of bacterial inclusion bodies (IBs) have revealed intriguing utilities of these protein particles. Since they combine mechanical stability and protein functionality, IBs have been already exploited in biocatalysis and explored for bottom-up topographical modification in tissue engineering. Being fully biocompatible and with tuneable bio-physical prope...
متن کاملLearning about protein solubility from bacterial inclusion bodies
The progressive solving of the conformation of aggregated proteins and the conceptual understanding of the biology of inclusion bodies in recombinant bacteria is providing exciting insights on protein folding and quality. Interestingly, newest data also show an unexpected functional and structural complexity of soluble recombinant protein species and picture the whole bacterial cell factory sce...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BioTechniques
سال: 2013
ISSN: 1940-9818,0736-6205
DOI: 10.2144/000113989